The Mouse ISG15 ELISA Kit is a pivotal tool for researchers studying immune response pathways and the role of ISG15 in cellular signaling. This article delves into the scientific background, applications, and methodology of this kit, complemented by trusted resources from educational and governmental websites.
ISG15, or Ubiquitin-like protein ISG15, is a small protein that becomes conjugated to target proteins in response to interferon stimulation. It plays a critical role in antiviral defense, immune modulation, and inflammation. Learn more about ISG15’s biochemical pathways at PubMed and the National Library of Medicine.
For detailed insights into interferon-stimulated genes (ISGs), explore resources at the National Institutes of Health and the National Center for Biotechnology Information.
Research Areas
The Mouse ISG15 ELISA Kit is extensively used in:
- Immunology Research: To study the immune response to viral infections. Resources from NIAID provide detailed insights into immunological pathways.
- Cancer Biology: Understanding the role of ISG15 in tumor suppression and immune evasion. Visit the National Cancer Institute for relevant studies.
- Infectious Disease Studies: Monitoring ISG15 levels during bacterial or viral infections. Explore research at the Centers for Disease Control and Prevention.
Sample Types
The ELISA kit is validated for plasma, serum, tissue lysates, and cell culture supernatants. For guidelines on sample collection and storage, refer to the World Health Organization and the Clinical Center at NIH.
Principles of ELISA
The enzyme-linked immunosorbent assay (ELISA) is a highly specific and sensitive method for detecting and quantifying proteins in a sample. Detailed tutorials on ELISA methods are available at NIAID.
Kit Components
- Antibody-coated plates.
- Standards and controls.
- Detection reagents.
- Washing and diluent buffers.
For standard laboratory practices, visit the Food and Drug Administration.
Steps in the Protocol
- Sample Preparation: Ensure proper collection and storage by following NIH’s biospecimen guidelines.
- Incubation: Add samples and standards to antibody-coated plates. Learn about assay optimization at NIST.
- Detection: Add enzyme-labeled antibodies and substrate for signal generation. Details on detection chemistry can be found at NSF.
- Analysis: Use a microplate reader to measure results. Refer to DOE resources for analytical techniques.
For troubleshooting tips and ELISA best practices, check resources from the Association of Biomolecular Resource Facilities.
Significance of the Mouse ISG15 ELISA Kit
Advancing Immunological Research
The kit enables precise quantification of ISG15, shedding light on its role in innate immunity. Explore ongoing research initiatives at the All of Us Research Program.
Bridging Basic and Translational Science
The Mouse ISG15 ELISA Kit facilitates the transition of basic research findings into clinical applications, a priority of NCATS.
Global Research Collaborations
Standardized ELISA kits promote consistency in research across laboratories worldwide. Investigate global health research initiatives at USAID.
Environmental and Public Health Impact
Monitoring ISG15 levels can provide insights into the impact of environmental factors on immune responses. Learn more at the Environmental Protection Agency and OSHA.
Conclusion
The Mouse ISG15 ELISA Kit is a vital resource for advancing our understanding of immune responses and their implications in health and disease. By leveraging resources from trusted organizations like Science.gov, researchers can make informed decisions and achieve reliable results.
For further reading, consult the HHS Research Grants, the National Academies, and the NIH RePORTER.
By facilitating discoveries in immunology and disease biology, the Mouse ISG15 ELISA Kit continues to be a cornerstone of modern scientific research.