Human Parkinson disease protein 7 (PARK7), also known as DJ-1, is a multifunctional protein involved in the protection of cells from oxidative stress and mitochondrial dysfunction—critical factors in the pathogenesis of Parkinson’s disease and other neurodegenerative conditions. To aid researchers in quantifying PARK7 levels in biological samples, the Human PARK7 ELISA Kit has become an indispensable tool. This article delves into its scientific importance, technical features, and applications, supported by authoritative academic and governmental sources.
What is PARK7?
PARK7 is a protein encoded by the PARK7 gene and plays a crucial role in cellular homeostasis. It is particularly vital in:
- Protecting neurons from oxidative stress
- Regulating mitochondrial function
- Acting as a molecular chaperone
To explore the molecular mechanisms of PARK7, visit the National Center for Biotechnology Information (NCBI).
Understanding the PARK7 ELISA Kit
The PARK7 ELISA Kit is designed to detect and quantify PARK7 in serum, plasma, cerebrospinal fluid, or cell lysates with high precision. This assay uses specific antibodies to ensure sensitivity and accuracy. For an introduction to ELISA technology, refer to the National Institutes of Health (NIH).
Applications of the PARK7 ELISA Kit
1. Parkinson’s Disease Research
Elevated or diminished PARK7 levels have been linked to Parkinson’s disease. Measuring PARK7 helps understand its role in disease progression and therapeutic interventions. Explore Parkinson’s research at the Michael J. Fox Foundation for Parkinson’s Research and the National Institute of Neurological Disorders and Stroke (NINDS).
2. Biomarker Discovery
PARK7 is a potential biomarker for oxidative stress-related disorders. Its detection can aid in early diagnosis and monitoring of neurodegenerative diseases. Learn more about biomarker development from the FDA Biomarker Qualification Program.
3. Toxicology Studies
PARK7 levels are studied in toxicology to evaluate the effects of environmental toxins and chemicals on neuronal health. The Environmental Protection Agency (EPA) provides additional information on toxicological research.
4. Mitochondrial Dysfunction Research
Given its role in mitochondrial regulation, PARK7 is integral to studies on mitochondrial diseases and age-related disorders. Visit the United Mitochondrial Disease Foundation (UMDF) for more insights.
Technical Features of the PARK7 ELISA Kit
1. High Sensitivity and Specificity
The kit’s antibodies are designed to specifically bind to PARK7, ensuring minimal cross-reactivity. For more details, see the National Cancer Institute (NCI).
2. Easy-to-Follow Protocols
The kit includes all necessary reagents, pre-coated plates, and detailed instructions for seamless execution. Learn about laboratory protocols from the Centers for Disease Control and Prevention (CDC).
3. Versatility
This ELISA Kit supports a range of sample types, making it suitable for diverse experimental setups. Refer to the National Institute of Standards and Technology (NIST) for standardization resources.
How to Use the PARK7 ELISA Kit
1. Sample Collection and Preparation
Proper handling of samples, including storage at optimal temperatures, is essential. Refer to the World Health Organization (WHO) for guidelines on sample collection.
2. Performing the Assay
Follow the step-by-step protocol provided in the kit, including incubation, washing, and signal detection steps. The Office of Research Integrity (ORI) offers tips on maintaining experimental integrity.
3. Data Analysis
Use the standard curve method to quantify PARK7 levels accurately. Visit the National Science Foundation (NSF) for tools and software recommendations for data analysis.
4. Troubleshooting
Issues such as inconsistent results or high background can occur. The National Institute of General Medical Sciences (NIGMS) provides resources to troubleshoot common ELISA challenges.
Advantages of the PARK7 ELISA Kit
- Accuracy: Provides precise and reproducible results, crucial for clinical and research applications. See MedlinePlus for a comparison of diagnostic tools.
- Speed: The streamlined protocol allows for rapid quantification of PARK7.
- Cost-Effectiveness: A more accessible alternative to advanced techniques like mass spectrometry. Explore funding opportunities for research at the NIH RePORTER.
Conclusion
The Human PARK7 ELISA Kit is a transformative tool in neurodegenerative and cellular biology research. Its applications span from studying Parkinson’s disease mechanisms to discovering biomarkers for oxidative stress. Researchers can rely on this robust and sensitive assay for their investigations. For more information, consult resources like the National Library of Medicine and the World Bank Open Knowledge Repository.
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