Year : 2012  |  Volume : 3  |  Issue : 1  |  Page : 48-52

Characterization of fungal biofilm-based catheter-related sepsis

1 Department of Microbiology, Govt. Medical College, Haldwani, Uttarakhand, India
2 Department of Microbiology, Jawaharlal Nehru Medical College, AMU, Aligarh, India
3 Department of Pediatrics, Jawaharlal Nehru Medical College, AMU, Aligarh, India
4 Department of Biochemistry, Govt. Medical College, Haldwani, Uttarakhand, India

Correspondence Address:
Monil Singhai
Assistant Professor, Department of Microbiology, Govt. Medical College, Haldwani, Uttarakhand - 263 139
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2229-5186.94317

Rights and Permissions

Background: Fungi most commonly associated with nosocomial septicemia are in the genus Candida. Attachment of yeasts to intravascular surfaces is the crucial step in initiating colonization by yeast cells, forming biofilms and resulting in disseminated infection depending on various factors. Aims: To study the rate and profile of fungal biofilms in catheter-related sepsis (CRS) and antifungal resistance among the clinical isolates of CRS was the aim of this study. Materials & Methods: In all, 135 hospitalized pediatric patients with peripheral intravascular catheters (IVCs) and clinical suspicion of nosocomial septicemia were studied. The yeast isolates causing CRS were identified and characterized, and antifungal susceptibility testing by microplate alamar blue method (minimum inhibitory concentration) was also done. The fungal biofilm formations were visualized by scanning electron microscopy and tube method. Results: 7.4% patients with IVC had CRS, majority being caused by Candida albicans biofilms. In vitro antifungal susceptibility testing of yeast isolates causing CRS demonstrated moderate to high level of resistance to fluconazole (70%). Voriconazole was the most optimum drug to cure such infections. Conclusion: This study illustrates the need for exploration of biofilm-based CRS (fungemia) in hospitalized patients and to design practical guidelines for their management (diagnosis and treatment).

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded380    
    Comments [Add]    
    Cited by others 1    

Recommend this journal