Year : 2012 | Volume
: 3 | Issue : 1 | Page : 57--62
LC-MS metabolomic analysis of environmental stressor impacts on the metabolite diversity in Nephthea spp.
Hedi Indra Januar, Endar Marraskuranto, Gintung Patantis, Ekowati Chasanah
Indonesian Research Center for Marine and Fisheries Products Processing and Biotechnology, Jl. KS Tubun Petamburan VI Slipi - Jakarta Pusat, Indonesia
Context: The soft coral Nephthea spp. is a source of terpenoid class that potentially has pharmaceutical properties. However, metabolite diversity and cytotoxic activity of this species are varied among coral reefs from various sites. Aim: To analyze the water quality in Nephthea spp. environment as a possible factor causing a difference in its metabolite diversity. Settings and Design: Nephthea spp. from seven sites were taken in October 2010 at the Alor District of Marine Protected Area, Indonesia. Materials and Methods: Water quality assessment was analyzed in situ and indexed by Canadian Council of Ministry Environment-Water Quality Index (CCME-WQI) method. Meanwhile, metabolite diversity was analyzed by a LC-MS metabolomic method, using C18 reversed phase and gradient water-acetonitrile system. Statistical Analysis Used: Spearman«SQ»s rho and regression analysis were applied to correlate the water quality index to ecological index (richness, diversity, and evenness) from LC-MS results. Results: The water quality index had a significant positive correlation and strong linear regression determinant to the total metabolite (R 2 = 0.704), particularly to semipolar metabolite richness (R 2 = 0.809), the area of terpenoid class in the organism. Conclusion: It can be concluded that water quality may serve as a major factor that affects the amount of richness in Nephthea spp. metabolites. When the water quality is lower, as environment stresses increases, it may affect the metabolite richness within direct disrupt of metabolite biosynthesis or indirect ecological means. Terpenoids are known as a soft coral antipredator (coral fishes), the amount of which depends on the water quality.
Hedi Indra Januar
Jl. KS Tubun Petamburan VI Slipi Jakarta Pusat 10260
|How to cite this article:|
Januar HI, Marraskuranto E, Patantis G, Chasanah E. LC-MS metabolomic analysis of environmental stressor impacts on the metabolite diversity in Nephthea spp. Chron Young Sci 2012;3:57-62
|How to cite this URL:|
Januar HI, Marraskuranto E, Patantis G, Chasanah E. LC-MS metabolomic analysis of environmental stressor impacts on the metabolite diversity in Nephthea spp. Chron Young Sci [serial online] 2012 [cited 2019 Sep 17 ];3:57-62
Available from: http://www.cysonline.org/article.asp?issn=2229-5186;year=2012;volume=3;issue=1;spage=57;epage=62;aulast=Januar;type=0